Detailed information of TA system TA157421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2537131..2537711
• Replicon: chromosome
• Accession: NZ_CP053675
• Organism: MAG: Ferrovum myxofaciens isolate MI1A

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A859ACQ7
• Locus tag: HO273_RS12660
• Protein ID: WP_031598903.1
• Coordinates: 2537385..2537711 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8F3IGG2
• Locus tag: HO273_RS12655
• Protein ID: WP_062188497.1
• Coordinates: 2537131..2537388 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO273_RS12630 (HO273_12125)	2532764..2533093	+	330	WP_197456570.1	hypothetical protein	-
HO273_RS12635 (HO273_12130)	2533706..2535166	+	1461	WP_273144676.1	IS21 family transposase	-
HO273_RS12640 (HO273_12135)	2535169..2535915	+	747	WP_273144679.1	IS21-like element helper ATPase IstB	-
HO273_RS12645 (HO273_12140)	2535905..2536177	+	273	WP_062188606.1	hypothetical protein	-
HO273_RS12650 (HO273_12145)	2536360..2536656	+	297	WP_156472717.1	site-specific recombinase resolvase	-
HO273_RS12655 (HO273_12150)	2537131..2537388	+	258	WP_062188497.1	hypothetical protein	Antitoxin
HO273_RS12660 (HO273_12155)	2537385..2537711	+	327	WP_031598903.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HO273_RS12665 (HO273_12160)	2537921..2538310	+	390	WP_273119893.1	cupin domain-containing protein	-
HO273_RS12670 (HO273_12165)	2538580..2539710	+	1131	WP_031598909.1	Fic family protein	-
HO273_RS12675 (HO273_12170)	2539775..2539987	+	213	WP_031598911.1	helix-turn-helix domain-containing protein	-
HO273_RS12680 (HO273_12175)	2540192..2540461	-	270	Protein_2489	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2528548..2539710	11162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11711.55 Da
• Isoelectric Point: 8.5354

>T157421 WP_031598903.1 NZ_CP053675:2537385-2537711 [Ferrovum myxofaciens]
MRRGDIYLVSLDPTEGREQRGSRPVLVVSPAEFNEATNLPVICPITNGGDFARRIGFAVPVTGIKTTGVVRCDQPRVLDF
GARNARKVDTLPASIMEEVLAKLATIFA

Nucleotide

• Length: 327 bp

>T157421 NZ_CP069303:c4127753-4127223 [Shinella sp. PSBB067]
ATGGCAGAAATAGGCGACACGCCGAAGCATTCTGAACCGCGGCTGTCCGCTCCCGTTCCTCTGAAGGCGGAGCATGACCT
GTCGGCGTTCGACTGCGGCGAGCCGGCGCTGAACGACTGGCTGCGCCAGCGGGCCTTGAAGAACGAAAGCCGGTTCTCGC
GCACCTATGTTGTGTGCGCGGGCAATCGGGTCGTCGGGTATTTCTGCATTTCGGCAGGATCGGTTGAACGTTCGGCTGCT
CCCGGCAAGGTGCGGCGTAACGCGCCCGACCTGATTCCGGTTTCGATAATTGGGCGGCTCGCTGTCAGCCTCGACCATGC
GGGCAGGGGCCTCGGCGCGGACCTGTTGTCGGACGCGCTGCGGCGGATTGCGCTGGCCTCGCAAAGCATCGGCATCGGGG
CCGTGATGGTCCAGGCGAAGGATGAAGCGGCAAAACGCTTCTATCTGCGCTGTGCGGAGTTCATCGAGTATCCAGAGGAC
AGCCGGACGCTGTTTCTACCCATCGAAACCGTTGTGGCTGGATTCGGTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9039.35 Da
• Isoelectric Point: 4.6646

>AT157421 WP_062188497.1 NZ_CP053675:2537131-2537388 [Ferrovum myxofaciens]
MHTTNLRKVGGSVMLAVPPAFLDQLHLQAGATVGVVVADGCLVVDPKPRPRYTLAELLAASDYSKPQSAEEREWVDAPAV
GDELV

Nucleotide

• Length: 258 bp

>AT157421 NZ_CP069303:c4128076-4127741 [Shinella sp. PSBB067]
ATGGCTAAAACTCGTCTCGCCACTTCCGAATCCTCCCGTTCCGCCGCTGTCGCGGCACGAGCCTCTGCGGCTGACACCAA
GGGCAGCATAAACCTGCGTATCGAAAGCGGCACTCGCCAACTCATCGACGACGCAGCAGCGGTCCTCGGCAAGACACGCA
CCGAGTTCATGGTTGAAAGCGCGCGGCGGCAGGCGGTGGATGTGCTGCTCGACCAGCGCCTGTTTACGCTCGATCCCGAG
CGTTACGATGCCTTCATGCAGGCGCTCGACAATCCGCCCGCTCCGGGACCGAAGCTCAAGGCGCTGCTACGCCGGACCCC
GGCATGGCAGAAATAG