Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52546..53071 Replicon plasmid unnamed3
Accession NZ_CP053668
Organism Klebsiella pneumoniae strain kpn-241

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HPK15_RS26880 Protein ID WP_013023785.1
Coordinates 52546..52851 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HPK15_RS26885 Protein ID WP_001568025.1
Coordinates 52853..53071 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK15_RS26850 (HPK15_27315) 48249..48875 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HPK15_RS26855 48872..49174 + 303 WP_004197636.1 hypothetical protein -
HPK15_RS26860 (HPK15_27325) 49622..50416 - 795 WP_004197635.1 site-specific integrase -
HPK15_RS26865 (HPK15_27335) 50614..51630 - 1017 WP_017899884.1 hypothetical protein -
HPK15_RS26870 (HPK15_27340) 51641..51955 - 315 WP_053389906.1 hypothetical protein -
HPK15_RS26875 (HPK15_27345) 51982..52377 - 396 WP_017899885.1 hypothetical protein -
HPK15_RS26880 (HPK15_27350) 52546..52851 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HPK15_RS26885 (HPK15_27355) 52853..53071 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HPK15_RS26890 (HPK15_27360) 53889..54179 - 291 WP_013023783.1 hypothetical protein -
HPK15_RS26895 (HPK15_27365) 54176..55303 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HPK15_RS26900 (HPK15_27370) 55337..56860 - 1524 WP_017899887.1 hypothetical protein -
HPK15_RS26905 (HPK15_27375) 57136..57915 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaIMP-4 / blaCTX-M-15 / blaTEM-1B - 1..97666 97666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T157411 WP_013023785.1 NZ_CP053668:c52851-52546 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T157411 NZ_CP069245:2036791-2038128 [Ralstonia pseudosolanacearum]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGCCTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACGGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGATCGCATCGACAGCGAGCCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCACGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCACCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGTCGCTGGATGCCGGGCAACGCCTGGATCGCGCGCCTGCCGCAGGAGGACTTCTGCCAGGCCACCGGCACGCCCGC
GCACCGCAAATACGAAAACGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTGCTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTATGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGCTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCGAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT157411 WP_001568025.1 NZ_CP053668:c53071-52853 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT157411 NZ_CP069245:2036542-2036787 [Ralstonia pseudosolanacearum]
ATGGAGTCCCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTGCTGCGCATCCTGCACGCGCTCGGCGCGCAGCTGACGCTGCGCACGTCCGCGCCCGCCGCCGCCGCGCCCGCCGAC
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References