Detailed information of TA system TA157410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 7100..7851
• Replicon: plasmid unnamed2
• Accession: NZ_CP053667
• Organism: Klebsiella pneumoniae strain kpn-241

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A486R6L7
• Locus tag: HPK15_RS25850
• Protein ID: WP_041165502.1
• Coordinates: 7100..7582 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A6S4YWG5
• Locus tag: HPK15_RS25855
• Protein ID: WP_012540170.1
• Coordinates: 7573..7851 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK15_RS25815 (HPK15_26250)	2510..2791	+	282	WP_207304475.1	hypothetical protein	-
HPK15_RS25820	2813..2968	+	156	Protein_4	IS5/IS1182 family transposase	-
HPK15_RS25825 (HPK15_26255)	3048..4016	+	969	WP_100112216.1	IS5-like element IS903 family transposase	-
HPK15_RS25830 (HPK15_26260)	4913..5149	+	237	WP_012540047.1	hypothetical protein	-
HPK15_RS25835 (HPK15_26265)	5244..5393	-	150	WP_223884741.1	bacteriocin immunity protein	-
HPK15_RS25840 (HPK15_26270)	5505..6338	-	834	WP_012540133.1	GIY-YIG nuclease family protein	-
HPK15_RS25845 (HPK15_26275)	6658..7062	-	405	WP_048293664.1	DUF2251 domain-containing protein	-
HPK15_RS25850 (HPK15_26280)	7100..7582	-	483	WP_041165502.1	GNAT family N-acetyltransferase	Toxin
HPK15_RS25855 (HPK15_26285)	7573..7851	-	279	WP_012540170.1	DUF1778 domain-containing protein	Antitoxin
HPK15_RS25860 (HPK15_26290)	8159..8695	+	537	WP_094986489.1	hypothetical protein	-
HPK15_RS25865 (HPK15_26295)	9700..10014	+	315	WP_023288299.1	hypothetical protein	-
HPK15_RS25870 (HPK15_26300)	10121..10410	-	290	Protein_14	Tn3 family transposase	-
HPK15_RS25875 (HPK15_26305)	10461..11213	-	753	WP_004729622.1	alpha/beta hydrolase	-
HPK15_RS25880 (HPK15_26310)	11454..12323	+	870	WP_001567390.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..129090	129090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17697.61 Da
• Isoelectric Point: 9.4948

>T157410 WP_041165502.1 NZ_CP053667:c7582-7100 [Klebsiella pneumoniae]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTQHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T157410 NZ_CP069245:362259-362672 [Ralstonia pseudosolanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10749.33 Da
• Isoelectric Point: 4.6722

>AT157410 WP_012540170.1 NZ_CP053667:c7851-7573 [Klebsiella pneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT157410 NZ_CP069245:362039-362272 [Ralstonia pseudosolanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Structures

Toxin

• AlphaFold DB: A0A486R6L7

Antitoxin

• AlphaFold DB: A0A6S4YWG5