Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5221559..5222184 Replicon chromosome
Accession NZ_CP053666
Organism Klebsiella pneumoniae strain kpn-241

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HPK15_RS25435 Protein ID WP_004187928.1
Coordinates 5221559..5221942 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HPK15_RS25440 Protein ID WP_004150355.1
Coordinates 5221942..5222184 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK15_RS25420 (5218925) 5218925..5219827 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HPK15_RS25425 (5219824) 5219824..5220459 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HPK15_RS25430 (5220456) 5220456..5221385 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HPK15_RS25435 (5221559) 5221559..5221942 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HPK15_RS25440 (5221942) 5221942..5222184 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HPK15_RS25445 (5222378) 5222378..5223295 + 918 WP_004187929.1 alpha/beta hydrolase -
HPK15_RS25450 (5223309) 5223309..5224250 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HPK15_RS25455 (5224295) 5224295..5224732 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HPK15_RS25460 (5224729) 5224729..5225589 - 861 WP_273604963.1 virulence factor BrkB family protein -
HPK15_RS25465 (5225583) 5225583..5226182 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T157409 WP_004187928.1 NZ_CP053666:c5221942-5221559 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T157409 NZ_CP069242:2378234-2378824 [Rhodococcus sp. USK13]
GTGAAACGTCTCGACCTGGTGGTCGGCTGCAACGGCGCCGGCAAGTCCACCTTCGTGGCGCTCACCCTCTCTCCCCTGCT
CCCGGCCCAACCGTTCGTCAATGCCGACGAGATCGCCAAACAACGATGGCCGGAGAATCCGGCTGCCCACTCCTACGACG
CGGCAGAGATCGCCGCGAAGACCCGGACGCACCTCCTGGCCCTGGGGAAATCGTTCATCGCCGAGACGGTGTTCTCCCAT
CCGTCGAAGCTGGAGCTGATCGACGAAGCGCGCACACACGAGTACACCGTCGTCTTGCACGTGGTGATGATTCCCGAGGA
GTTAGCGGTCCAGCGTGTGCGCTACCGGGTGCGAGCCGGCGGGCACGATGTGCCCGAAGACAAGATCCGCCAGCGATACC
GAAGACTGTGGGGTCTCGTCGCTCAGGCATTCCCACGTTGCGATCATGCGACCGTGTACGACAACAGCTCGATCACAGGC
CCGCGCATCGTCGCACAGTTCACCGACGGACACCTCGTCGGCGCAGCCACCTGGCCTGCATGGACACCCGCAGAACTGAC
CTCGCACACGAGCCGAAGTGAGCAACTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT157409 WP_004150355.1 NZ_CP053666:c5222184-5221942 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT157409 NZ_CP069242:2377854-2378237 [Rhodococcus sp. USK13]
ATGCAAAGCAGTGCGGACAAGGTCACCCGCCTGGCAGCCGACCTGGTAGCCAGCGCCATGGCCGAGGGAGAACGGCAGCA
CCGCACAGGACGCCAGCAACTCGAACACTGGGCCCGGGTGGGGCGTGCGGTCTCGAGCCGGCAGACCGCGGCTCGCCGCC
GAGTAGAAGACGCTCTCGCCGGCCGGGTGCCGATCGGTGAACTCGGCACCGAGGAAGGCACGGTGTTCAACGCCGAGATC
AACGCGGCGATCGAGGAACAACTGGCCACCGCCCATTACGGCACCGCGCTCGCGGCCCAGGGAATCACCACCGTCGCGCT
GTCCGAGGATGGTCAGATCGTCGAGTACCGTCCCGACGGCACACACGCAGTCCTGCCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References