Detailed information of TA system TA157406

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4646205..4646908
• Replicon: chromosome
• Accession: NZ_CP053666
• Organism: Klebsiella pneumoniae strain kpn-241

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2Z3WYB7
• Locus tag: HPK15_RS22705
• Protein ID: WP_031593213.1
• Coordinates: 4646205..4646546 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2Z3WZC0
• Locus tag: HPK15_RS22710
• Protein ID: WP_032410777.1
• Coordinates: 4646567..4646908 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK15_RS22675 (4641249)	4641249..4641515	+	267	WP_004178415.1	hypothetical protein	-
HPK15_RS22680 (4641515)	4641515..4642187	+	673	Protein_4444	DUF4400 domain-containing protein	-
HPK15_RS22685 (4642198)	4642198..4643082	+	885	WP_004192285.1	RES domain-containing protein	-
HPK15_RS22690 (4643281)	4643281..4643469	-	189	Protein_4446	transposase	-
HPK15_RS22695 (4643486)	4643486..4644597	-	1112	Protein_4447	IS3 family transposase	-
HPK15_RS22700 (4644965)	4644965..4645972	-	1008	WP_049017154.1	restriction endonuclease	-
HPK15_RS22705 (4646205)	4646205..4646546	-	342	WP_031593213.1	TA system toxin CbtA family protein	Toxin
HPK15_RS22710 (4646567)	4646567..4646908	-	342	WP_032410777.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPK15_RS22715 (4646919)	4646919..4647461	-	543	WP_100116935.1	DNA repair protein RadC	-
HPK15_RS22720 (4647474)	4647474..4647914	-	441	WP_001616563.1	antirestriction protein	-
HPK15_RS22725 (4647945)	4647945..4648766	-	822	WP_094986557.1	DUF932 domain-containing protein	-
HPK15_RS22730 (4648887)	4648887..4649360	-	474	WP_031593204.1	hypothetical protein	-
HPK15_RS22735 (4649432)	4649432..4649884	-	453	WP_031593203.1	hypothetical protein	-
HPK15_RS22740 (4649920)	4649920..4650636	-	717	WP_094986556.1	WYL domain-containing protein	-
HPK15_RS22745 (4650880)	4650880..4651755	-	876	WP_094986555.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4635360..4678213	42853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12736.66 Da
• Isoelectric Point: 8.0324

>T157406 WP_031593213.1 NZ_CP053666:c4646546-4646205 [Klebsiella pneumoniae]
MKTLPATTPQAATLCLSPVSVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T157406 NZ_CP069219:918684-918893 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12765.40 Da
• Isoelectric Point: 7.0269

>AT157406 WP_032410777.1 NZ_CP053666:c4646908-4646567 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT157406 NZ_CP069219:918384-918677 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A2Z3WYB7

Antitoxin

• AlphaFold DB: A0A2Z3WZC0