Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4606433..4607243 Replicon chromosome
Accession NZ_CP053666
Organism Klebsiella pneumoniae strain kpn-241

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag HPK15_RS22485 Protein ID WP_023279404.1
Coordinates 4606433..4606966 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HPK15_RS22490 Protein ID WP_002887278.1
Coordinates 4606977..4607243 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK15_RS22480 (4605264) 4605264..4606385 + 1122 WP_002887282.1 cupin domain-containing protein -
HPK15_RS22485 (4606433) 4606433..4606966 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
HPK15_RS22490 (4606977) 4606977..4607243 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HPK15_RS22495 (4607346) 4607346..4608779 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
HPK15_RS22500 (4608769) 4608769..4609452 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HPK15_RS22505 (4609624) 4609624..4611009 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
HPK15_RS22510 (4611027) 4611027..4611371 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T157405 WP_023279404.1 NZ_CP053666:c4606966-4606433 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T157405 NZ_CP069219:c779864-779412 [Staphylococcus epidermidis]
GTGGGTAAATACGAAGATTTGATGATTAAATACGACCATTTACCTATTACAGAAACTAAATATATGCCGGACTTCATGTC
CGGTCTGTATTTGGACGGGGAAATACTCATCAATGATAACAGGAGTGTTAGGCAAAAATTAGAAATATTAGCAGAAGAAA
TCGCTCATCACGAAATAACATATGGAAATATATTGAATATAAATGATTTTCAAAACAATAAATATGAATTAAAAGCACGA
CGGTTAGCTTTTGAAATGTTGGTTGGCTTAGATGACTTAGTAATAGCTCATAATGAAGGTGTTAAAAACACATATGAACT
TTCTGAATTTTTTGAAGTGACAGAACAATTCATCAAAGATTGTTTAAAACACTACAAAACGAAACACGGAAATAAGGTCA
AACATGATAATTATTTAATCACTTTTGAACCATTAAATGTAAAGGAGATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT157405 WP_002887278.1 NZ_CP053666:c4607243-4606977 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT157405 NZ_CP069219:c780188-779877 [Staphylococcus epidermidis]
ATGAGTATTGGTAACAGAATAAAAAAACGTAGAAAAGAAATGAAAATGTCAGCAGATAAGTTGGCAGAAAAAATAGGTAA
AAATCGCGCAACAGTTTACAGATATGAAAAAGATGAAATAGAAAATATGCCTTACGATGTTTTAGAACCTATAGCAGAAG
CATTAAATGTTTCTCCCGCTTATCTTATGGGATGGGAAGAAAAAGAAAAAACTGAAACGGTAGCAGCACATTTAGATTAT
TCAGATTTAACAGAGGAGGAACAAAAAGAGGTAGAGAATTTTATTGATTACATAAGAAACAGAAGAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References