Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4006405..4007024 Replicon chromosome
Accession NZ_CP053666
Organism Klebsiella pneumoniae strain kpn-241

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HPK15_RS19610 Protein ID WP_002892050.1
Coordinates 4006806..4007024 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HPK15_RS19605 Protein ID WP_002892066.1
Coordinates 4006405..4006779 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK15_RS19595 (4001556) 4001556..4002749 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HPK15_RS19600 (4002772) 4002772..4005919 + 3148 Protein_3844 multidrug efflux RND transporter permease subunit AcrB -
HPK15_RS19605 (4006405) 4006405..4006779 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HPK15_RS19610 (4006806) 4006806..4007024 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HPK15_RS19615 (4007187) 4007187..4007753 + 567 WP_002892030.1 maltose O-acetyltransferase -
HPK15_RS19620 (4007725) 4007725..4007865 - 141 WP_004147370.1 hypothetical protein -
HPK15_RS19625 (4007886) 4007886..4008356 + 471 WP_002892026.1 YlaC family protein -
HPK15_RS19630 (4008331) 4008331..4009782 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HPK15_RS19635 (4009883) 4009883..4010581 + 699 WP_002892021.1 GNAT family protein -
HPK15_RS19640 (4010578) 4010578..4010718 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HPK15_RS19645 (4010718) 4010718..4010981 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T157404 WP_002892050.1 NZ_CP053666:4006806-4007024 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T157404 NZ_CP069215:1877672-1878133 [Staphylococcus epidermidis]
ATGGGGTTATATGAAAAAATGTTAATAGAACATGATTATATAGAAGTCAGAGAGACAAATGTTATGCCTAATAACCTACA
CGGTCTATGGTTAGGTGATTTAATTTTAATTAAGCGAAACTTATCAGAAATACGAAAAGCCGAAGTATTATACGAAGAAC
TAGCACATCATAAACTTACATATGGAAACATCTTAGATCAATCTAAAGATATAAATCGCAAATTTGAAAACTACGCTAGG
CGTTACGGATATGAAGCTGCCCTACCTTTGCGTATTATTGTGGAGGCGCATAACTATGGTGTTAGTAACTTATATGAATT
AGCTGAATATGTTCAATTAAGTGAAGAGTACATAGCAGAAATATTGAAACATTACAAAAATAAATATGGTATTGGAACTC
ACTATGAAGAATACCTAATTACATTTGATCCGTTAAGGGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT157404 WP_002892066.1 NZ_CP053666:4006405-4006779 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT157404 NZ_CP069215:1877337-1877660 [Staphylococcus epidermidis]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATAAAACAACTACGTAAAGAGAACAATATAAATTTAACTGATTTTGCAAC
TAAAATAGGTGTTAATAAATCTACATTATCAAGATATGAAAATGGTAGTAGAAAAATACCTATGGAAGATATAGCTGAAA
TCGCAAATGCATTGAATGTTACCCCAGAAAGTTTATTACTAAAAAATAAACAACCAGAAACTGAAATACAACATCGTGCA
GCTCATCTTGAAGCCGAATTAACAGATGATGAATGGAAACGTGTATTAGATTATGCAGATTATATAAGAAGCAAACGCAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References