Detailed information of TA system TA157381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 269684..270209
• Replicon: plasmid unnamed
• Accession: NZ_CP053663
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: HPK14_RS27290
• Protein ID: WP_001159868.1
• Coordinates: 269904..270209 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: HPK14_RS27285
• Protein ID: WP_000813634.1
• Coordinates: 269684..269902 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS27260 (264819)	264819..265040	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPK14_RS27265 (265223)	265223..266155	+	933	Protein_325	type I restriction-modification system subunit M	-
HPK14_RS27270 (266211)	266211..266908	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
HPK14_RS27275 (267045)	267045..267977	-	933	WP_000991832.1	S-4TM family putative pore-forming effector	-
HPK14_RS27280 (267981)	267981..268976	-	996	WP_000246636.1	hypothetical protein	-
HPK14_RS27285 (269684)	269684..269902	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HPK14_RS27290 (269904)	269904..270209	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
HPK14_RS27295 (270210)	270210..271016	+	807	WP_000016982.1	site-specific integrase	-
HPK14_RS27300 (271790)	271790..272545	+	756	WP_000852146.1	replication initiation protein RepE	-
HPK14_RS27305 (273133)	273133..274299	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSFO-1 / blaTEM-1B / aac(3)-IId / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14 / tet(B)	senB	1..340412	340412
	-	inside	IScluster/Tn	-	-	258660..266908	8248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T157381 WP_001159868.1 NZ_CP053663:269904-270209 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T157381 NZ_CP069198:6476399-6476677 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT157381 WP_000813634.1 NZ_CP053663:269684-269902 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT157381 NZ_CP069198:6476689-6476994 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2