Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3837491..3838109 Replicon chromosome
Accession NZ_CP053662
Organism Escherichia coli strain eco-241

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HPK14_RS18905 Protein ID WP_001291435.1
Coordinates 3837891..3838109 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HPK14_RS18900 Protein ID WP_000344800.1
Coordinates 3837491..3837865 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK14_RS18890 (3832580) 3832580..3833773 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HPK14_RS18895 (3833796) 3833796..3836945 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
HPK14_RS18900 (3837491) 3837491..3837865 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HPK14_RS18905 (3837891) 3837891..3838109 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HPK14_RS18910 (3838281) 3838281..3838832 + 552 WP_000102559.1 maltose O-acetyltransferase -
HPK14_RS18915 (3838948) 3838948..3839418 + 471 WP_000136192.1 YlaC family protein -
HPK14_RS18920 (3839582) 3839582..3841132 + 1551 Protein_3704 cyclic-guanylate-specific phosphodiesterase PdeB -
HPK14_RS18925 (3841174) 3841174..3841527 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HPK14_RS18935 (3841906) 3841906..3842217 + 312 WP_000409911.1 MGMT family protein -
HPK14_RS18940 (3842248) 3842248..3842820 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T157369 WP_001291435.1 NZ_CP053662:3837891-3838109 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T157369 NZ_CP069185:c2719795-2719454 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT157369 WP_000344800.1 NZ_CP053662:3837491-3837865 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT157369 NZ_CP069185:c2720025-2719795 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References