Detailed information of TA system TA157368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3347040..3347745
• Replicon: chromosome
• Accession: NZ_CP053662
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HPK14_RS16455
• Protein ID: WP_000539521.1
• Coordinates: 3347040..3347426 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HPK14_RS16460
• Protein ID: WP_001280945.1
• Coordinates: 3347416..3347745 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS16435 (3343044)	3343044..3343670	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HPK14_RS16440 (3343667)	3343667..3344782	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
HPK14_RS16445 (3344893)	3344893..3345276	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HPK14_RS16450 (3345489)	3345489..3346814	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HPK14_RS16455 (3347040)	3347040..3347426	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPK14_RS16460 (3347416)	3347416..3347745	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HPK14_RS16465 (3347815)	3347815..3349143	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
HPK14_RS16470 (3349151)	3349151..3351499	-	2349	Protein_3231	cyclic diguanylate phosphodiesterase	-
HPK14_RS16475 (3351677)	3351677..3352588	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T157368 WP_000539521.1 NZ_CP053662:3347040-3347426 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T157368 NZ_CP069185:1759382-1759726 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCGGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAAGAAACTTATTTAAAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT157368 WP_001280945.1 NZ_CP053662:3347416-3347745 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT157368 NZ_CP069185:1759033-1759365 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6