Detailed information of TA system TA157367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3099839..3100623
• Replicon: chromosome
• Accession: NZ_CP053662
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HPK14_RS15280
• Protein ID: WP_000613626.1
• Coordinates: 3100129..3100623 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HPK14_RS15275
• Protein ID: WP_001110447.1
• Coordinates: 3099839..3100132 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS15265 (3094989)	3094989..3095948	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HPK14_RS15270 (3096521)	3096521..3099706	+	3186	WP_000827427.1	ribonuclease E	-
HPK14_RS15275 (3099839)	3099839..3100132	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HPK14_RS15280 (3100129)	3100129..3100623	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HPK14_RS15285 (3100718)	3100718..3101671	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
HPK14_RS15290 (3101683)	3101683..3103326	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
HPK14_RS15295 (3103392)	3103392..3104336	-	945	WP_273680256.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HPK14_RS15300 (3104336)	3104336..3105433	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T157367 WP_000613626.1 NZ_CP053662:3100129-3100623 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T157367 NZ_CP069185:445881-446156 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT157367 WP_001110447.1 NZ_CP053662:3099839-3100132 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT157367 NZ_CP069185:445622-445888 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6