Detailed information of TA system TA157349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2314984..2315959
• Replicon: chromosome
• Accession: NZ_CP053656
• Organism: Bacillus cereus strain CTMA_1571

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HPG46_RS11990
• Protein ID: WP_142952205.1
• Coordinates: 2314984..2315721 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: HPG46_RS11995
• Protein ID: WP_000588712.1
• Coordinates: 2315834..2315959 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPG46_RS11960	2310057..2310767	+	711	WP_086419968.1	class I SAM-dependent methyltransferase	-
HPG46_RS11965	2310887..2311294	+	408	WP_000072479.1	VOC family protein	-
HPG46_RS11970	2311402..2311494	+	93	Protein_2273	methyltransferase	-
HPG46_RS11975	2311616..2313301	-	1686	WP_171855987.1	alpha-keto acid decarboxylase family protein	-
HPG46_RS11980	2313408..2313890	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
HPG46_RS11985	2314058..2314795	+	738	WP_086419970.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
HPG46_RS11990	2314984..2315721	+	738	WP_142952205.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HPG46_RS11995	2315834..2315959	+	126	WP_000588712.1	hypothetical protein	Antitoxin
HPG46_RS12000	2316035..2316196	+	162	WP_000852626.1	hypothetical protein	-
HPG46_RS12005	2316233..2316514	-	282	WP_086419972.1	hypothetical protein	-
HPG46_RS12010	2316804..2317032	-	229	Protein_2281	hypothetical protein	-
HPG46_RS12015	2317371..2317547	+	177	WP_171855988.1	hypothetical protein	-
HPG46_RS12020	2317691..2319160	+	1470	WP_171855989.1	beta-Ala-His dipeptidase	-
HPG46_RS12025	2319743..2320213	-	471	WP_171855990.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28339.02 Da
• Isoelectric Point: 8.2705

>T157349 WP_142952205.1 NZ_CP053656:2314984-2315721 [Bacillus cereus]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVLEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T157349 NZ_CP069171:146828-147271 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT157349 WP_000588712.1 NZ_CP053656:2315834-2315959 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT157349 NZ_CP069171:146601-146831 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1