Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 250677..251319 Replicon chromosome
Accession NZ_CP053656
Organism Bacillus cereus strain CTMA_1571

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag HPG46_RS01415 Protein ID WP_000635963.1
Coordinates 250969..251319 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HPG46_RS01410 Protein ID WP_000004570.1
Coordinates 250677..250964 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPG46_RS01385 245993..246955 + 963 WP_171855602.1 UV DNA damage repair endonuclease UvsE -
HPG46_RS01390 246948..247520 - 573 WP_147791996.1 rhomboid family intramembrane serine protease -
HPG46_RS01395 247613..247972 + 360 WP_000635040.1 holo-ACP synthase -
HPG46_RS01400 248129..249079 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
HPG46_RS01405 249198..250367 + 1170 WP_171855603.1 alanine racemase -
HPG46_RS01410 250677..250964 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HPG46_RS01415 250969..251319 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HPG46_RS01420 251387..253555 + 2169 WP_171855604.1 RNA-binding transcriptional accessory protein -
HPG46_RS01425 253613..253729 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HPG46_RS01430 253925..254383 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T157348 WP_000635963.1 NZ_CP053656:250969-251319 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T157348 NZ_CP069171:c53057-52641 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT157348 WP_000004570.1 NZ_CP053656:250677-250964 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT157348 NZ_CP069171:c53284-53054 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References