Detailed information of TA system TA157346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1075957..1076596
• Replicon: chromosome
• Accession: NZ_CP053655
• Organism: Bifidobacterium breve strain 1101A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A133L4T2
• Locus tag: HPH06_RS04605
• Protein ID: WP_021649636.1
• Coordinates: 1075957..1076313 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HPH06_RS04610
• Protein ID: WP_230578351.1
• Coordinates: 1076300..1076596 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPH06_RS04575 (HPH06_04520)	1071143..1072054	+	912	WP_258900921.1	ABC transporter ATP-binding protein	-
HPH06_RS04580 (HPH06_04525)	1072251..1072877	+	627	WP_003829226.1	30S ribosomal protein S4	-
HPH06_RS04585 (HPH06_04530)	1073214..1073996	-	783	WP_015438747.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HPH06_RS04590 (HPH06_04535)	1074139..1074624	-	486	WP_003829229.1	cytidine deaminase	-
HPH06_RS04595 (HPH06_04540)	1074717..1075073	+	357	WP_003833231.1	SdpI family protein	-
HPH06_RS04600 (HPH06_04545)	1075178..1075780	+	603	WP_016463212.1	transglutaminase family protein	-
HPH06_RS04605 (HPH06_04550)	1075957..1076313	-	357	WP_021649636.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HPH06_RS04610 (HPH06_04555)	1076300..1076596	-	297	WP_230578351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HPH06_RS04615 (HPH06_04560)	1077130..1077531	+	402	WP_258900922.1	PadR family transcriptional regulator	-
HPH06_RS04620 (HPH06_04565)	1077528..1078190	+	663	WP_258900923.1	hypothetical protein	-
HPH06_RS04625 (HPH06_04570)	1078402..1079637	-	1236	WP_258900924.1	tyrosine-type recombinase/integrase	-
HPH06_RS04630 (HPH06_04575)	1080154..1080360	-	207	WP_258900925.1	hypothetical protein	-
HPH06_RS04635 (HPH06_04580)	1080415..1081533	-	1119	WP_258900926.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13332.27 Da
• Isoelectric Point: 8.9603

>T157346 WP_021649636.1 NZ_CP053655:c1076313-1075957 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T157346 NZ_CP069170:c5395861-5395478 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10967.47 Da
• Isoelectric Point: 4.2896

>AT157346 WP_230578351.1 NZ_CP053655:c1076596-1076300 [Bifidobacterium breve]
MMAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT157346 NZ_CP069170:c5396103-5395861 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A133L4T2