Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32135..32772 Replicon plasmid pEh65_5
Accession NZ_CP053648
Organism Enterobacter hormaechei strain Eh65

Toxin (Protein)


Gene name vagD Uniprot ID A0A9E6WXZ4
Locus tag HPC69_RS24680 Protein ID WP_032662169.1
Coordinates 32135..32545 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A9E6WTF5
Locus tag HPC69_RS24685 Protein ID WP_032662167.1
Coordinates 32542..32772 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPC69_RS24660 (HPC69_24605) 27501..27851 + 351 WP_032662182.1 DUF305 domain-containing protein -
HPC69_RS24665 (HPC69_24610) 28037..28945 - 909 WP_032662179.1 HNH endonuclease -
HPC69_RS24670 (HPC69_24620) 29492..30514 - 1023 WP_032662174.1 helicase UvrD -
HPC69_RS24675 (HPC69_24625) 30499..32061 - 1563 WP_032662171.1 AAA family ATPase -
HPC69_RS24680 (HPC69_24630) 32135..32545 - 411 WP_032662169.1 PIN domain-containing protein Toxin
HPC69_RS24685 (HPC69_24635) 32542..32772 - 231 WP_032662167.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HPC69_RS24690 (HPC69_24640) 33314..33604 + 291 WP_013087126.1 hypothetical protein -
HPC69_RS24695 (HPC69_24645) 33735..34265 + 531 WP_013087127.1 hypothetical protein -
HPC69_RS24700 (HPC69_24650) 34284..35060 + 777 WP_015572054.1 site-specific integrase -
HPC69_RS24705 (HPC69_24655) 35262..35519 + 258 Protein_36 site-specific integrase -
HPC69_RS24710 (HPC69_24660) 35763..36773 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B - 1..79223 79223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14729.03 Da        Isoelectric Point: 5.7351

>T157340 WP_032662169.1 NZ_CP053648:c32545-32135 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 411 bp

>T157340 NZ_CP069170:2009564-2009666 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8645.77 Da        Isoelectric Point: 4.4692

>AT157340 WP_032662167.1 NZ_CP053648:c32772-32542 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Download         Length: 231 bp

>AT157340 NZ_CP069170:c2009673-2009528 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References