Detailed information of TA system TA157338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93050..94673
• Replicon: plasmid pEh65_2
• Accession: NZ_CP053645
• Organism: Enterobacter hormaechei strain Eh65

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: HPC69_RS23050
• Protein ID: WP_000088044.1
• Coordinates: 93348..94673 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: HPC69_RS23045
• Protein ID: WP_012477377.1
• Coordinates: 93050..93343 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPC69_RS23025 (HPC69_22960)	88849..89241	+	393	WP_000341066.1	hypothetical protein	-
HPC69_RS23030 (HPC69_22965)	89900..90556	+	657	WP_000462754.1	trypsin-like serine protease	-
HPC69_RS23035 (HPC69_22970)	90759..91256	+	498	WP_000340139.1	membrane protein	-
HPC69_RS23040 (HPC69_22975)	91261..92649	+	1389	WP_012695445.1	hypothetical protein	-
HPC69_RS23045 (HPC69_22980)	93050..93343	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
HPC69_RS23050 (HPC69_22985)	93348..94673	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
HPC69_RS23055 (HPC69_22990)	94734..94940	+	207	WP_000134171.1	hypothetical protein	-
HPC69_RS23060 (HPC69_22995)	95041..95451	+	411	WP_000985909.1	hypothetical protein	-
HPC69_RS23065 (HPC69_23000)	95464..95988	+	525	WP_001805097.1	hypothetical protein	-
HPC69_RS23070 (HPC69_23005)	96170..97174	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / blaSHV-12 / dfrA16 / blaCTX-M-9 / mcr-9	-	1..295898	295898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T157338 WP_000088044.1 NZ_CP053645:93348-94673 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T157338 NZ_CP069170:c874258-873911 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT157338 WP_012477377.1 NZ_CP053645:93050-93343 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT157338 NZ_CP069170:c873911-873612 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7