Detailed information of TA system TA157337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37143..37940
• Replicon: plasmid pEh65_1
• Accession: NZ_CP053644
• Organism: Enterobacter hormaechei strain Eh65

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HPC69_RS22190
• Protein ID: WP_172754447.1
• Coordinates: 37437..37940 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HPC69_RS22185
• Protein ID: WP_016582626.1
• Coordinates: 37143..37412 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPC69_RS22160 (HPC69_22085)	32473..33813	-	1341	WP_006812582.1	DnaB-like helicase C-terminal domain-containing protein	-
HPC69_RS22165 (HPC69_22090)	33874..34599	-	726	WP_058652463.1	hypothetical protein	-
HPC69_RS22170 (HPC69_22095)	34863..35660	+	798	WP_077990606.1	DUF2971 domain-containing protein	-
HPC69_RS22175 (HPC69_22100)	35702..36055	-	354	WP_160956455.1	hypothetical protein	-
HPC69_RS22180 (HPC69_22105)	36061..36726	-	666	WP_023315962.1	AAA family ATPase	-
HPC69_RS22185 (HPC69_22110)	37143..37412	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HPC69_RS22190 (HPC69_22115)	37437..37940	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
HPC69_RS22195 (HPC69_22120)	37968..38132	-	165	WP_224743081.1	hypothetical protein	-
HPC69_RS22200 (HPC69_22125)	38125..38376	-	252	WP_000147960.1	hypothetical protein	-
HPC69_RS22205 (HPC69_22130)	38378..39070	-	693	WP_006812500.1	membrane protein	-
HPC69_RS22210 (HPC69_22135)	39084..39407	-	324	WP_000064173.1	hypothetical protein	-
HPC69_RS22215 (HPC69_22140)	39482..40270	-	789	WP_040110304.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114699	114699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T157337 WP_172754447.1 NZ_CP053644:37437-37940 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T157337 NZ_CP069170:c481480-480995 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT157337 WP_016582626.1 NZ_CP053644:37143-37412 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT157337 NZ_CP069170:c481770-481477 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3