Detailed information of TA system TA157324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 301743..302339
• Replicon: chromosome
• Accession: NZ_CP053643
• Organism: Enterobacter hormaechei strain Eh65

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: HPC69_RS01435
• Protein ID: WP_023315213.1
• Coordinates: 302037..302339 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HPC69_RS01430
• Protein ID: WP_023315212.1
• Coordinates: 301743..302030 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPC69_RS01425 (HPC69_01430)	300115..301746	+	1632	WP_058652456.1	Na/Pi cotransporter family protein	-
HPC69_RS01430 (HPC69_01435)	301743..302030	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
HPC69_RS01435 (HPC69_01440)	302037..302339	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPC69_RS01440 (HPC69_01445)	302537..303409	+	873	WP_045346556.1	23S rRNA pseudouridine(2604) synthase RluF	-
HPC69_RS01445 (HPC69_01450)	303410..303682	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
HPC69_RS01450 (HPC69_01455)	303733..304677	-	945	WP_058652454.1	ketopantoate/pantoate/pantothenate transporter PanS	-
HPC69_RS01455 (HPC69_01460)	304771..306120	-	1350	WP_003860344.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T157324 WP_023315213.1 NZ_CP053643:c302339-302037 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T157324 NZ_CP069159:33780-34121 [Citrobacter sp. R56]
ATGTGGGATGTTGAAACAACGGACGTTTTCGACAGGTGGTTTGACGAACAAACTGAAGCACTCAAGGAGGACATTCTCGC
CGCTATGATTATTTTGTCCGAATATGGCCCCCAATTGGGAAGACCGTTTGCGGATACGGTGAACGACTCCGCTTTTTCGA
ACATGAAAGAGTTACGCATTCAGCATCAAGGTAACCCAATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGTCATGGCATC
GTGTTGTGCGCTGGAGATAAAACGGGGTTAAACGAGAAGAAATTCTATAAGGATATGATTAAGTTTGCAGATGCTGAGTA
CAGAAAACATCTTAATAAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT157324 WP_023315212.1 NZ_CP053643:c302030-301743 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT157324 NZ_CP069159:34131-34430 [Citrobacter sp. R56]
ATGGCTACCCTCAAAGAGTTGATGGCTAAACAAACCCCGGAAAGTCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCA
CCAGGCTATTGCATTAAGTATGTTAAGGGAAGAACTTAACCTGTCTCAGGCAGAACTGGCCGCGGCAATGGGCGTTAAGC
AGCCAACCATTGCAAAAATGGAACAGGCTGAAAATGATCCTCGCCTCTCTACGTTGAAACGTTACGTAGCAGCGCTGGGT
GGCGAGTTAAGTATTGATGTTAAGTTGCCCACAGGTAAGAGAGTGGCGTTCCATATTTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8