Detailed information of TA system TA157300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2683113..2683889
• Replicon: chromosome
• Accession: NZ_CP053639
• Organism: Staphylococcus aureus strain 14732

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: HA036_RS13340
• Protein ID: WP_000031108.1
• Coordinates: 2683113..2683265 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: HA036_RS13345
• Protein ID: WP_031806872.1
• Coordinates: 2683290..2683889 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA036_RS13325	2679193..2680014	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
HA036_RS13330	2680477..2681862	-	1386	WP_000116224.1	class II fumarate hydratase	-
HA036_RS13335	2682058..2682453	-	396	WP_000901021.1	hypothetical protein	-
HA036_RS13340	2683113..2683265	-	153	WP_000031108.1	hypothetical protein	Toxin
HA036_RS13345	2683290..2683889	-	600	WP_031806872.1	glucosamine-6-phosphate isomerase	Antitoxin
HA036_RS13350	2684048..2684518	-	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
HA036_RS13355	2684523..2685650	-	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
HA036_RS13360	2685801..2686523	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
HA036_RS13365	2686516..2687973	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T157300 WP_000031108.1 NZ_CP053639:c2683265-2683113 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T157300 NZ_CP069133:c1408184-1407990 [Escherichia coli BW25113]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22333.43 Da
• Isoelectric Point: 5.1445

>AT157300 WP_031806872.1 NZ_CP053639:c2683889-2683290 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQASVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT157300 NZ_CP069133:1407813-1407991 [Escherichia coli BW25113]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: X5E2E6