Detailed information of TA system TA157289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 45863..46392
• Replicon: chromosome
• Accession: NZ_CP053639
• Organism: Staphylococcus aureus strain 14732

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HA036_RS00395
• Protein ID: WP_000621175.1
• Coordinates: 45863..46225 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HA036_RS00400
• Protein ID: WP_000948331.1
• Coordinates: 46222..46392 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA036_RS00375	42842..43612	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
HA036_RS00380	43587..44066	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HA036_RS00385	44068..44394	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HA036_RS00390	44513..45514	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HA036_RS00395	45863..46225	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HA036_RS00400	46222..46392	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HA036_RS00405	46477..47625	-	1149	WP_001281145.1	alanine racemase	-
HA036_RS00410	47691..48050	-	360	WP_000581200.1	holo-ACP synthase	-
HA036_RS00415	48054..48545	-	492	WP_001205910.1	PH domain-containing protein	-
HA036_RS00420	48532..50115	-	1584	WP_001294626.1	PH domain-containing protein	-
HA036_RS00425	50108..50587	-	480	WP_001287088.1	hypothetical protein	-
HA036_RS00430	50795..51355	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T157289 WP_000621175.1 NZ_CP053639:c46225-45863 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T157289 NZ_CP069132:4342721-4342894 [Escherichia coli BW25113]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT157289 WP_000948331.1 NZ_CP053639:c46392-46222 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT157289 NZ_CP069132:4342397-4342693 [Escherichia coli BW25113]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1