Detailed information of TA system TA157266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2612568..2613082
• Replicon: chromosome
• Accession: NZ_CP053634
• Organism: Staphylococcus aureus strain 14638

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: HA038_RS13000
• Protein ID: WP_000581792.1
• Coordinates: 2612873..2613082 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: HA038_RS12995
• Protein ID: WP_000138932.1
• Coordinates: 2612568..2612861 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA038_RS12975	2607831..2608472	+	642	WP_000483153.1	thiamine phosphate synthase	-
HA038_RS12980	2608558..2609430	+	873	WP_000725802.1	membrane protein insertase YidC	-
HA038_RS12985	2610243..2610890	-	648	WP_001187630.1	HD domain-containing protein	-
HA038_RS12990	2610918..2612402	-	1485	WP_000571549.1	cardiolipin synthase	-
HA038_RS12995	2612568..2612861	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
HA038_RS13000	2612873..2613082	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
HA038_RS13005	2613210..2613347	+	138	WP_000828354.1	hypothetical protein	-
HA038_RS13010	2613611..2614813	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
HA038_RS13015	2615131..2616201	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
HA038_RS13020	2616216..2617574	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T157266 WP_000581792.1 NZ_CP053634:2612873-2613082 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T157266 NZ_CP069132:c242722-242444 [Escherichia coli BW25113]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT157266 WP_000138932.1 NZ_CP053634:2612568-2612861 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT157266 NZ_CP069132:c242985-242725 [Escherichia coli BW25113]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13