Detailed information of TA system TA157246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1463321..1463862
• Replicon: chromosome
• Accession: NZ_CP053627
• Organism: Xylella taiwanensis strain PLS229

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Z9JGT1
• Locus tag: PLS229_RS06410
• Protein ID: WP_038273166.1
• Coordinates: 1463539..1463862 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Z9JGL9
• Locus tag: PLS229_RS06405
• Protein ID: WP_038273164.1
• Coordinates: 1463321..1463542 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLS229_RS06355	1458897..1459121	-	225	WP_081755494.1	hypothetical protein	-
PLS229_RS06360	1459142..1459348	-	207	WP_038273075.1	hypothetical protein	-
PLS229_RS06365	1459351..1459668	-	318	WP_051482395.1	hypothetical protein	-
PLS229_RS06370	1459941..1460528	-	588	WP_038273076.1	hypothetical protein	-
PLS229_RS06375	1460725..1460868	-	144	WP_160165233.1	hypothetical protein	-
PLS229_RS06380	1460988..1461188	-	201	WP_038273077.1	hypothetical protein	-
PLS229_RS06385	1461237..1461500	-	264	WP_162814095.1	hypothetical protein	-
PLS229_RS06390	1461504..1461665	-	162	WP_160165236.1	hypothetical protein	-
PLS229_RS06395	1461808..1462284	+	477	WP_152536672.1	DUF3693 domain-containing protein	-
PLS229_RS06400	1462195..1463035	-	841	Protein_1240	site-specific integrase	-
PLS229_RS06405	1463321..1463542	+	222	WP_038273164.1	antitoxin MazE family protein	Antitoxin
PLS229_RS06410	1463539..1463862	+	324	WP_038273166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PLS229_RS06415	1463963..1465111	-	1149	WP_069636227.1	hypothetical protein	-
PLS229_RS06420	1465111..1465452	-	342	WP_038273089.1	DUF2523 domain-containing protein	-
PLS229_RS06425	1465456..1466523	-	1068	WP_152536670.1	hypothetical protein	-
PLS229_RS06430	1466991..1467209	-	219	WP_051482398.1	phage coat protein	-
PLS229_RS06435	1467218..1467424	-	207	WP_038273092.1	hypothetical protein	-
PLS229_RS06440	1467427..1467720	-	294	WP_038273093.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1447227..1476393	29166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11807.97 Da
• Isoelectric Point: 9.1188

>T157246 WP_038273166.1 NZ_CP053627:1463539-1463862 [Xylella taiwanensis]
MTQRGDLVTVSLQGDYGKPRPALILQSDLLMELDSVVLCPVTSELRNAIFRITVEPNIANGLRTLSQIMVDKISTLPRSK
ISKPFGHLNDERIKAVEKALLLIVGII

Nucleotide

• Length: 324 bp

>T157246 NZ_CP069078:3821803-3822213 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8490.65 Da
• Isoelectric Point: 8.0827

>AT157246 WP_038273164.1 NZ_CP053627:1463321-1463542 [Xylella taiwanensis]
MAFIHEPTSKVARHRKQMRAAGLRPVQFWVPDTRTPEFAAEIRSQCRSLKNDPEEADALRFTEEAITHIEGWK

Nucleotide

• Length: 222 bp

>AT157246 NZ_CP069078:3821528-3821806 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: Z9JGT1

Antitoxin

• AlphaFold DB: Z9JGL9