Detailed information of TA system TA157213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 328379..329016
• Replicon: chromosome
• Accession: NZ_CP053609
• Organism: Burkholderia sp. MBR-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1G5N4H6
• Locus tag: MBR110_RS01560
• Protein ID: WP_034192973.1
• Coordinates: 328379..328780 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MBR110_RS01565
• Protein ID: WP_011882846.1
• Coordinates: 328780..329016 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBR110_RS01550	325756..326892	+	1137	WP_045578623.1	alginate lyase family protein	-
MBR110_RS01555	326923..328311	+	1389	WP_011882848.1	L-serine ammonia-lyase	-
MBR110_RS01560	328379..328780	-	402	WP_034192973.1	type II toxin-antitoxin system VapC family toxin	Toxin
MBR110_RS01565	328780..329016	-	237	WP_011882846.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MBR110_RS01570	329295..330998	+	1704	WP_059621851.1	thiamine pyrophosphate-binding protein	-
MBR110_RS01575	330995..332122	+	1128	WP_059621849.1	hypothetical protein	-
MBR110_RS01580	332088..332294	-	207	WP_181709139.1	hypothetical protein	-
MBR110_RS01585	332355..333389	-	1035	WP_011882843.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14848.01 Da
• Isoelectric Point: 5.1682

>T157213 WP_034192973.1 NZ_CP053609:c328780-328379 [Burkholderia sp. MBR-1]
MPRYMLDTNMCIYLMKSQPEQVAKRFAQCYTGDVVMSAITCAELEYGVTACAAPARERRHLAALIEDIPVAPFDAAAAQA
YGPVRDATRAWKKDHLDKLIAAHAVSLDVVLVTNDERDFTSYPGLRLENWLND

Nucleotide

• Length: 402 bp

>T157213 NZ_CP069078:c1570853-1570452 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9021.21 Da
• Isoelectric Point: 7.2056

>AT157213 WP_011882846.1 NZ_CP053609:c329016-328780 [Burkholderia sp. MBR-1]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPARRPLTQVLKKFAKFAPDFMAEGRGDHEQAEREGL

Nucleotide

• Length: 237 bp

>AT157213 NZ_CP069078:c1571107-1570850 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: A0A1G5N4H6