Detailed information of TA system TA157199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4476227..4477041
• Replicon: chromosome
• Accession: NZ_CP053607
• Organism: Escherichia coli strain NEB5-alpha_F'Iq

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HO392_RS21590
• Protein ID: WP_001054376.1
• Coordinates: 4476784..4477041 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HO392_RS21585
• Protein ID: WP_001309181.1
• Coordinates: 4476227..4476772 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO392_RS21560	4471918..4473231	-	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-
HO392_RS21565	4473243..4473521	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HO392_RS21570	4473518..4474639	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HO392_RS23395	4474884..4475000	-	117	Protein_4196	VOC family protein	-
HO392_RS21575	4475038..4475256	-	219	Protein_4197	hypothetical protein	-
HO392_RS21580	4475425..4476171	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HO392_RS21585	4476227..4476772	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HO392_RS21590	4476784..4477041	-	258	WP_001054376.1	hypothetical protein	Toxin
HO392_RS21595	4477418..4477663	-	246	Protein_4201	GNAT family N-acetyltransferase	-
HO392_RS21600	4477779..4479019	+	1241	Protein_4202	DNA helicase	-
HO392_RS21605	4479602..4480582	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HO392_RS21610	4480647..4481753	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4473518..4486397	12879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T157199 WP_001054376.1 NZ_CP053607:c4477041-4476784 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T157199 NZ_CP069078:710415-710810 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT157199 WP_001309181.1 NZ_CP053607:c4476772-4476227 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT157199 NZ_CP069078:710161-710415 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8