Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 18424..19103 | Replicon | plasmid F' |
| Accession | NZ_CP053606 | ||
| Organism | Escherichia coli strain NEB_Turbo | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | HO393_RS21885 | Protein ID | WP_000854672.1 |
| Coordinates | 18424..18765 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | HO393_RS21890 | Protein ID | WP_000070395.1 |
| Coordinates | 18786..19103 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HO393_RS21860 | 13701..14102 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| HO393_RS21865 | 14141..15196 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| HO393_RS21870 | 15484..16587 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| HO393_RS21875 | 16599..17852 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| HO393_RS21885 | 18424..18765 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| HO393_RS21890 | 18786..19103 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| HO393_RS21895 | 19122..19343 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| HO393_RS21900 | 19352..19828 | - | 477 | WP_000811693.1 | RadC family protein | - |
| HO393_RS21905 | 19844..20302 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| HO393_RS21910 | 20400..20639 | - | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| HO393_RS21915 | 20716..21183 | - | 468 | WP_001547765.1 | hypothetical protein | - |
| HO393_RS21920 | 21206..21649 | - | 444 | WP_000824223.1 | hypothetical protein | - |
| HO393_RS21925 | 21649..21876 | - | 228 | WP_001548158.1 | hypothetical protein | - |
| HO393_RS21930 | 21872..22063 | - | 192 | Protein_30 | DeoR family transcriptional regulator | - |
| HO393_RS21935 | 22280..23101 | - | 822 | WP_000197389.1 | DUF945 domain-containing protein | - |
| HO393_RS21940 | 23193..24056 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC | 1..231547 | 231547 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T157154 WP_000854672.1 NZ_CP053606:c18765-18424 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T157154 NZ_CP069077:1544409-1545230 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT157154 WP_000070395.1 NZ_CP053606:c19103-18786 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT157154 NZ_CP069077:1545223-1545459 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|