Detailed information of TA system TA157154

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 18424..19103
• Replicon: plasmid F'
• Accession: NZ_CP053606
• Organism: Escherichia coli strain NEB_Turbo

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: HO393_RS21885
• Protein ID: WP_000854672.1
• Coordinates: 18424..18765 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: HO393_RS21890
• Protein ID: WP_000070395.1
• Coordinates: 18786..19103 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO393_RS21860	13701..14102	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
HO393_RS21865	14141..15196	-	1056	WP_000749863.1	phosphoporin PhoE	-
HO393_RS21870	15484..16587	+	1104	WP_001285288.1	glutamate 5-kinase	-
HO393_RS21875	16599..17852	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HO393_RS21885	18424..18765	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HO393_RS21890	18786..19103	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HO393_RS21895	19122..19343	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HO393_RS21900	19352..19828	-	477	WP_000811693.1	RadC family protein	-
HO393_RS21905	19844..20302	-	459	WP_000211838.1	antirestriction protein	-
HO393_RS21910	20400..20639	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HO393_RS21915	20716..21183	-	468	WP_001547765.1	hypothetical protein	-
HO393_RS21920	21206..21649	-	444	WP_000824223.1	hypothetical protein	-
HO393_RS21925	21649..21876	-	228	WP_001548158.1	hypothetical protein	-
HO393_RS21930	21872..22063	-	192	Protein_30	DeoR family transcriptional regulator	-
HO393_RS21935	22280..23101	-	822	WP_000197389.1	DUF945 domain-containing protein	-
HO393_RS21940	23193..24056	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..231547	231547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T157154 WP_000854672.1 NZ_CP053606:c18765-18424 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T157154 NZ_CP069077:1544409-1545230 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT157154 WP_000070395.1 NZ_CP053606:c19103-18786 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT157154 NZ_CP069077:1545223-1545459 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684