Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4241318..4241815 Replicon chromosome
Accession NZ_CP053605
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HO393_RS20370 Protein ID WP_001173343.1
Coordinates 4241642..4241815 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HO393_RS20365 Protein ID WP_000398619.1
Coordinates 4241318..4241614 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS20345 4238049..4238768 - 720 WP_000611288.1 two-component system response regulator DcuR -
HO393_RS20350 4238765..4240396 - 1632 WP_001216477.1 sensor histidine kinase -
HO393_RS20355 4240577..4240807 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HO393_RS20360 4240819..4241091 + 273 WP_000405651.1 N-acetyltransferase -
HO393_RS20365 4241318..4241614 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HO393_RS20370 4241642..4241815 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HO393_RS20375 4241934..4243451 - 1518 WP_001295074.1 lysine--tRNA ligase -
HO393_RS20380 4243688..4245145 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T157149 WP_001173343.1 NZ_CP053605:4241642-4241815 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T157149 NZ_CP069077:1170935-1171816 [Mycobacterium tuberculosis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT157149 WP_000398619.1 NZ_CP053605:4241318-4241614 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT157149 NZ_CP069077:1170315-1170938 [Mycobacterium tuberculosis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References