Detailed information of TA system TA157142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2650051..2651488
• Replicon: chromosome
• Accession: NZ_CP053605
• Organism: Escherichia coli strain NEB_Turbo

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: HO393_RS12855
• Protein ID: WP_000155570.1
• Coordinates: 2650051..2651124 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: HO393_RS12860
• Protein ID: WP_000461704.1
• Coordinates: 2651117..2651488 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO393_RS12840	2645484..2647673	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
HO393_RS12845	2647670..2649286	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
HO393_RS12850	2649646..2649909	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
HO393_RS12855	2650051..2651124	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
HO393_RS12860	2651117..2651488	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
HO393_RS12865	2651843..2652706	+	864	WP_000787597.1	GTPase family protein	-
HO393_RS12870	2652798..2653619	+	822	WP_000197391.1	DUF945 domain-containing protein	-
HO393_RS12875	2653836..2654537	+	702	WP_000189409.1	WYL domain-containing protein	-
HO393_RS12880	2654578..2654814	+	237	WP_001144029.1	hypothetical protein	-
HO393_RS12885	2654814..2655257	+	444	WP_000824222.1	hypothetical protein	-
HO393_RS12890	2655281..2655748	+	468	WP_000702495.1	hypothetical protein	-
HO393_RS12895	2655973..2656287	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T157142 WP_000155570.1 NZ_CP053605:2650051-2651124 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T157142 NZ_CP069077:721810-722217 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT157142 WP_000461704.1 NZ_CP053605:2651117-2651488 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT157142 NZ_CP069077:721553-721813 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4