Detailed information of TA system TA157125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 366136..366754
• Replicon: chromosome
• Accession: NZ_CP053605
• Organism: Escherichia coli strain NEB_Turbo

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: HO393_RS01750
• Protein ID: WP_001290581.1
• Coordinates: 366136..366354 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: HO393_RS01755
• Protein ID: WP_000344800.1
• Coordinates: 366380..366754 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO393_RS01715	361425..361997	+	573	WP_000779842.1	YbaY family lipoprotein	-
HO393_RS01720	362028..362339	-	312	WP_000409911.1	MGMT family protein	-
HO393_RS01730	362718..363071	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
HO393_RS01735	363113..364663	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
HO393_RS01740	364827..365297	-	471	WP_000136192.1	YlaC family protein	-
HO393_RS01745	365413..365964	-	552	WP_000102564.1	maltose O-acetyltransferase	-
HO393_RS01750	366136..366354	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
HO393_RS01755	366380..366754	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
HO393_RS01760	367300..370449	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
HO393_RS01765	370472..371665	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T157125 WP_001290581.1 NZ_CP053605:c366354-366136 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T157125 NZ_CP069076:3539591-3539935 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT157125 WP_000344800.1 NZ_CP053605:c366754-366380 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT157125 NZ_CP069076:3539932-3540261 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5