Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2168762..2169593 Replicon chromosome
Accession NZ_CP053604
Organism Escherichia coli strain NEB10-beta

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HO394_RS10705 Protein ID WP_000854814.1
Coordinates 2169219..2169593 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag HO394_RS10700 Protein ID WP_001285584.1
Coordinates 2168762..2169130 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO394_RS10685 2166429..2167961 + 1533 WP_001350525.1 hypothetical protein -
HO394_RS10690 2167958..2168404 + 447 WP_000187523.1 RadC family protein -
HO394_RS10695 2168467..2168688 + 222 WP_000692323.1 DUF987 domain-containing protein -
HO394_RS10700 2168762..2169130 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HO394_RS10705 2169219..2169593 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HO394_RS10710 2169590..2169784 + 195 WP_000988600.1 hypothetical protein -
HO394_RS10715 2169797..2169910 + 114 WP_001161660.1 DUF957 domain-containing protein -
HO394_RS10720 2170199..2170327 - 129 Protein_2095 transposase domain-containing protein -
HO394_RS10725 2170399..2170581 + 183 WP_001016348.1 hypothetical protein -
HO394_RS10730 2170682..2171011 - 330 WP_000450409.1 DUF496 family protein -
HO394_RS10735 2171183..2172241 - 1059 WP_001200891.1 FUSC family protein -
HO394_RS10740 2172439..2172912 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
HO394_RS10745 2173031..2174197 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T157111 WP_000854814.1 NZ_CP053604:2169219-2169593 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T157111 NZ_CP069076:2795753-2796178 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT157111 WP_001285584.1 NZ_CP053604:2168762-2169130 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT157111 NZ_CP069076:2795526-2795747 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References