Detailed information of TA system TA157100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1600523..1601161
• Replicon: chromosome
• Accession: NZ_CP053604
• Organism: Escherichia coli strain NEB10-beta

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HO394_RS07810
• Protein ID: WP_000813794.1
• Coordinates: 1600523..1600699 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HO394_RS07815
• Protein ID: WP_001270286.1
• Coordinates: 1600745..1601161 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO394_RS07790	1596142..1597356	-	1215	WP_001326689.1	BenE family transporter YdcO	-
HO394_RS07795	1597409..1597945	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HO394_RS07800	1598018..1599979	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HO394_RS07805	1600071..1600301	-	231	WP_000494244.1	YncJ family protein	-
HO394_RS07810	1600523..1600699	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HO394_RS07815	1600745..1601161	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HO394_RS07820	1601240..1602646	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HO394_RS07825	1602891..1604036	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HO394_RS07830	1604054..1605067	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HO394_RS07835	1605068..1606009	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T157100 WP_000813794.1 NZ_CP053604:1600523-1600699 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T157100 NZ_CP069076:1962435-1962683 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT157100 WP_001270286.1 NZ_CP053604:1600745-1601161 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT157100 NZ_CP069076:1962223-1962435 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• PDB: 6HPB