Detailed information of TA system TA157094

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 237992..238671
• Replicon: chromosome
• Accession: NZ_CP053604
• Organism: Escherichia coli strain NEB10-beta

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: HO394_RS01130
• Protein ID: WP_000854672.1
• Coordinates: 237992..238333 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: HO394_RS01135
• Protein ID: WP_000070395.1
• Coordinates: 238354..238671 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO394_RS01105	233269..233670	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
HO394_RS01110	233709..234764	-	1056	WP_000749863.1	phosphoporin PhoE	-
HO394_RS01115	235052..236155	+	1104	WP_001285288.1	glutamate 5-kinase	-
HO394_RS01120	236167..237420	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HO394_RS01130	237992..238333	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HO394_RS01135	238354..238671	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HO394_RS01140	238690..238911	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HO394_RS01145	238920..239396	-	477	WP_000811693.1	RadC family protein	-
HO394_RS01150	239412..239870	-	459	WP_000211838.1	antirestriction protein	-
HO394_RS01155	239968..240207	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HO394_RS01160	240284..240751	-	468	WP_001547765.1	hypothetical protein	-
HO394_RS01165	240774..241217	-	444	WP_000824223.1	hypothetical protein	-
HO394_RS01170	241217..241444	-	228	WP_001548158.1	hypothetical protein	-
HO394_RS01175	241440..241631	-	192	Protein_226	DeoR family transcriptional regulator	-
HO394_RS01180	241848..242669	-	822	WP_000197389.1	DUF945 domain-containing protein	-
HO394_RS01185	242761..243624	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T157094 WP_000854672.1 NZ_CP053604:c238333-237992 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T157094 NZ_CP069076:c1389177-1388884 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT157094 WP_000070395.1 NZ_CP053604:c238671-238354 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT157094 NZ_CP069076:c1389443-1389174 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684