Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3018274..3018928 Replicon chromosome
Accession NZ_CP053603
Organism Escherichia coli strain Dam/Dcm

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HO395_RS14575 Protein ID WP_000244777.1
Coordinates 3018274..3018681 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HO395_RS14580 Protein ID WP_000354046.1
Coordinates 3018662..3018928 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO395_RS14555 3014231..3015964 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
HO395_RS14560 3015970..3016680 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HO395_RS14565 3016705..3017601 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HO395_RS14570 3017713..3018234 + 522 WP_001055874.1 flavodoxin FldB -
HO395_RS14575 3018274..3018681 - 408 WP_000244777.1 toxin CptA Toxin
HO395_RS14580 3018662..3018928 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HO395_RS14585 3019171..3020151 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HO395_RS14590 3020347..3021006 - 660 WP_000250274.1 hemolysin III family protein -
HO395_RS14595 3021170..3021481 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HO395_RS14600 3021526..3022959 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HO395_RS14605 3023016..3023759 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T157079 WP_000244777.1 NZ_CP053603:c3018681-3018274 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157079 NZ_CP069076:c694030-693617 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157079 WP_000354046.1 NZ_CP053603:c3018928-3018662 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157079 NZ_CP069076:c694263-694027 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References