Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1493331..1493969 Replicon chromosome
Accession NZ_CP053603
Organism Escherichia coli strain Dam/Dcm

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HO395_RS07225 Protein ID WP_000813794.1
Coordinates 1493331..1493507 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HO395_RS07230 Protein ID WP_001270286.1
Coordinates 1493553..1493969 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO395_RS07205 1488950..1490164 - 1215 WP_001326689.1 BenE family transporter YdcO -
HO395_RS07210 1490217..1490753 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HO395_RS07215 1490826..1492787 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HO395_RS07220 1492879..1493109 - 231 WP_000494244.1 YncJ family protein -
HO395_RS07225 1493331..1493507 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HO395_RS07230 1493553..1493969 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HO395_RS07235 1494048..1495454 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HO395_RS07240 1495699..1496844 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HO395_RS07245 1496862..1497875 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HO395_RS07250 1497876..1498817 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T157063 WP_000813794.1 NZ_CP053603:1493331-1493507 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T157063 NZ_CP069075:c3144372-3143980 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT157063 WP_001270286.1 NZ_CP053603:1493553-1493969 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT157063 NZ_CP069075:c3144584-3144369 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References