Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 482982..483600 Replicon chromosome
Accession NZ_CP053603
Organism Escherichia coli strain Dam/Dcm

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HO395_RS02355 Protein ID WP_001291435.1
Coordinates 482982..483200 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HO395_RS02360 Protein ID WP_000344800.1
Coordinates 483226..483600 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO395_RS02320 478271..478843 + 573 WP_000779842.1 YbaY family lipoprotein -
HO395_RS02325 478874..479185 - 312 WP_000409911.1 MGMT family protein -
HO395_RS02335 479564..479917 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HO395_RS02340 479959..481509 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HO395_RS02345 481673..482143 - 471 WP_000136192.1 YlaC family protein -
HO395_RS02350 482259..482810 - 552 WP_000102564.1 maltose O-acetyltransferase -
HO395_RS02355 482982..483200 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HO395_RS02360 483226..483600 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HO395_RS02365 484146..487295 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HO395_RS02370 487318..488511 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T157052 WP_001291435.1 NZ_CP053603:c483200-482982 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T157052 NZ_CP069075:2815437-2815862 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT157052 WP_000344800.1 NZ_CP053603:c483600-483226 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT157052 NZ_CP069075:2815210-2815431 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References