Detailed information of TA system TA157051

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263329..264008
• Replicon: chromosome
• Accession: NZ_CP053603
• Organism: Escherichia coli strain Dam/Dcm

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: HO395_RS01240
• Protein ID: WP_000854672.1
• Coordinates: 263329..263670 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: HO395_RS01245
• Protein ID: WP_000070395.1
• Coordinates: 263691..264008 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO395_RS01215	258606..259007	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
HO395_RS01220	259046..260101	-	1056	WP_000749863.1	phosphoporin PhoE	-
HO395_RS01225	260389..261492	+	1104	WP_001285288.1	glutamate 5-kinase	-
HO395_RS01230	261504..262757	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
HO395_RS01240	263329..263670	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HO395_RS01245	263691..264008	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HO395_RS01250	264027..264248	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HO395_RS01255	264257..264733	-	477	WP_000811693.1	RadC family protein	-
HO395_RS01260	264749..265207	-	459	WP_000211838.1	antirestriction protein	-
HO395_RS01265	265305..265544	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HO395_RS01270	265621..266088	-	468	WP_001547765.1	hypothetical protein	-
HO395_RS01275	266111..266554	-	444	WP_000824223.1	hypothetical protein	-
HO395_RS01280	266554..266781	-	228	WP_001548158.1	hypothetical protein	-
HO395_RS01285	266777..266968	-	192	Protein_248	DeoR family transcriptional regulator	-
HO395_RS01290	267185..268006	-	822	WP_000197389.1	DUF945 domain-containing protein	-
HO395_RS01295	268098..268961	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	263109..281972	18863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T157051 WP_000854672.1 NZ_CP053603:c263670-263329 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T157051 NZ_CP069075:c2412303-2411986 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT157051 WP_000070395.1 NZ_CP053603:c264008-263691 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT157051 NZ_CP069075:c2412515-2412300 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684