Detailed information of TA system TA157048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4443040..4443854
• Replicon: chromosome
• Accession: NZ_CP053602
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HO396_RS21435
• Protein ID: WP_001054376.1
• Coordinates: 4443597..4443854 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HO396_RS21430
• Protein ID: WP_001309181.1
• Coordinates: 4443040..4443585 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO396_RS21405	4438731..4440044	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HO396_RS21410	4440056..4440334	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HO396_RS21415	4440331..4441452	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HO396_RS21420	4441851..4442069	-	219	Protein_4170	hypothetical protein	-
HO396_RS21425	4442238..4442984	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HO396_RS21430	4443040..4443585	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HO396_RS21435	4443597..4443854	-	258	WP_001054376.1	hypothetical protein	Toxin
HO396_RS21440	4444231..4444476	-	246	Protein_4174	GNAT family N-acetyltransferase	-
HO396_RS21445	4444846..4445832	+	987	Protein_4175	hypothetical protein	-
HO396_RS21450	4446415..4447395	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HO396_RS21455	4447460..4448566	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T157048 WP_001054376.1 NZ_CP053602:c4443854-4443597 [Escherichia coli BL21(DE3)]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T157048 NZ_CP069075:2261712-2262110 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT157048 WP_001309181.1 NZ_CP053602:c4443585-4443040 [Escherichia coli BL21(DE3)]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT157048 NZ_CP069075:2261484-2261711 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8