Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2872574..2873228 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HO396_RS13955 Protein ID WP_000244783.1
Coordinates 2872574..2872981 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HO396_RS13960 Protein ID WP_000354046.1
Coordinates 2872962..2873228 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS13935 2868531..2870264 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HO396_RS13940 2870270..2870980 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HO396_RS13945 2871005..2871901 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HO396_RS13950 2872013..2872534 + 522 WP_001055874.1 flavodoxin FldB -
HO396_RS13955 2872574..2872981 - 408 WP_000244783.1 protein YgfX Toxin
HO396_RS13960 2872962..2873228 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HO396_RS13965 2873471..2874451 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HO396_RS13970 2874647..2875306 - 660 WP_000250274.1 hemolysin III family protein -
HO396_RS13975 2875470..2875781 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HO396_RS13980 2875826..2877259 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
HO396_RS13985 2877316..2878059 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T157044 WP_000244783.1 NZ_CP053602:c2872981-2872574 [Escherichia coli BL21(DE3)]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157044 NZ_CP069075:c2203532-2203236 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157044 WP_000354046.1 NZ_CP053602:c2873228-2872962 [Escherichia coli BL21(DE3)]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157044 NZ_CP069075:c2203780-2203529 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References