Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1464647..1465285 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HO396_RS07250 Protein ID WP_000813794.1
Coordinates 1464647..1464823 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HO396_RS07255 Protein ID WP_001270286.1
Coordinates 1464869..1465285 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS07230 1460266..1461480 - 1215 WP_001326689.1 BenE family transporter YdcO -
HO396_RS07235 1461533..1462069 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HO396_RS07240 1462142..1464103 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HO396_RS07245 1464195..1464425 - 231 WP_000494244.1 YncJ family protein -
HO396_RS07250 1464647..1464823 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HO396_RS07255 1464869..1465285 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HO396_RS07260 1465364..1466770 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HO396_RS07265 1467015..1468160 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HO396_RS07270 1468178..1469191 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HO396_RS07275 1469192..1470133 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T157033 WP_000813794.1 NZ_CP053602:1464647-1464823 [Escherichia coli BL21(DE3)]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T157033 NZ_CP069075:1244299-1244673 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT157033 WP_001270286.1 NZ_CP053602:1464869-1465285 [Escherichia coli BL21(DE3)]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT157033 NZ_CP069075:1244105-1244302 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References