Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 450434..451052 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HO396_RS02120 Protein ID WP_001291435.1
Coordinates 450434..450652 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HO396_RS02125 Protein ID WP_000344800.1
Coordinates 450678..451052 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS02085 445723..446295 + 573 WP_000779826.1 lipoprotein -
HO396_RS02090 446326..446637 - 312 WP_000409911.1 MGMT family protein -
HO396_RS02100 447016..447369 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HO396_RS02105 447411..448961 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HO396_RS02110 449125..449595 - 471 WP_000136192.1 YlaC family protein -
HO396_RS02115 449711..450262 - 552 WP_000102564.1 maltose O-acetyltransferase -
HO396_RS02120 450434..450652 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HO396_RS02125 450678..451052 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HO396_RS02130 451598..454747 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HO396_RS02135 454770..455963 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T157026 WP_001291435.1 NZ_CP053602:c450652-450434 [Escherichia coli BL21(DE3)]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T157026 NZ_CP069075:c760072-759689 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTTGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT157026 WP_000344800.1 NZ_CP053602:c451052-450678 [Escherichia coli BL21(DE3)]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT157026 NZ_CP069075:c760322-760167 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References