Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 414773..415610 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag HO396_RS01945 Protein ID WP_000227786.1
Coordinates 414773..415315 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HO396_RS01950 Protein ID WP_001297137.1
Coordinates 415299..415610 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS01925 410303..411214 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
HO396_RS01930 411382..411873 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HO396_RS01935 412001..413365 - 1365 WP_001000978.1 MFS transporter -
HO396_RS01940 413782..414717 + 936 WP_001297127.1 sel1 repeat family protein -
HO396_RS01945 414773..415315 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
HO396_RS01950 415299..415610 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HO396_RS01955 415795..416685 - 891 WP_000971336.1 heme o synthase -
HO396_RS01960 416697..417026 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HO396_RS01965 417026..417640 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HO396_RS01970 417630..419621 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HO396_RS01975 419643..420590 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T157025 WP_000227786.1 NZ_CP053602:c415315-414773 [Escherichia coli BL21(DE3)]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T157025 NZ_CP069075:724455-724862 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT157025 WP_001297137.1 NZ_CP053602:c415610-415299 [Escherichia coli BL21(DE3)]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT157025 NZ_CP069075:724198-724458 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References