Detailed information of TA system TA157022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4412202..4413016
• Replicon: chromosome
• Accession: NZ_CP053601
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HO397_RS21245
• Protein ID: WP_001054376.1
• Coordinates: 4412759..4413016 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HO397_RS21240
• Protein ID: WP_001309181.1
• Coordinates: 4412202..4412747 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO397_RS21215	4407893..4409206	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HO397_RS21220	4409218..4409496	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HO397_RS21225	4409493..4410614	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HO397_RS21230	4411013..4411231	-	219	Protein_4132	hypothetical protein	-
HO397_RS21235	4411400..4412146	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HO397_RS21240	4412202..4412747	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HO397_RS21245	4412759..4413016	-	258	WP_001054376.1	hypothetical protein	Toxin
HO397_RS21250	4413393..4413638	-	246	Protein_4136	GNAT family N-acetyltransferase	-
HO397_RS21255	4414008..4414994	+	987	Protein_4137	hypothetical protein	-
HO397_RS21260	4415577..4416557	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HO397_RS21265	4416622..4417728	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4370613..4422372	51759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T157022 WP_001054376.1 NZ_CP053601:c4413016-4412759 [Escherichia coli BL21]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T157022 NZ_CP069075:709659-710060 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGATGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT157022 WP_001309181.1 NZ_CP053601:c4412747-4412202 [Escherichia coli BL21]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT157022 NZ_CP069075:709417-709662 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8