Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2841736..2842390 Replicon chromosome
Accession NZ_CP053601
Organism Escherichia coli BL21

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HO397_RS13760 Protein ID WP_000244783.1
Coordinates 2841736..2842143 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HO397_RS13765 Protein ID WP_000354046.1
Coordinates 2842124..2842390 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO397_RS13740 2837693..2839426 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HO397_RS13745 2839432..2840142 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HO397_RS13750 2840167..2841063 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HO397_RS13755 2841175..2841696 + 522 WP_001055874.1 flavodoxin FldB -
HO397_RS13760 2841736..2842143 - 408 WP_000244783.1 protein YgfX Toxin
HO397_RS13765 2842124..2842390 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HO397_RS13770 2842633..2843613 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HO397_RS13775 2843809..2844468 - 660 WP_000250274.1 hemolysin III family protein -
HO397_RS13780 2844632..2844943 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HO397_RS13785 2844988..2846421 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
HO397_RS13790 2846478..2847221 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T157018 WP_000244783.1 NZ_CP053601:c2842143-2841736 [Escherichia coli BL21]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157018 NZ_CP069075:c646813-646400 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157018 WP_000354046.1 NZ_CP053601:c2842390-2842124 [Escherichia coli BL21]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157018 NZ_CP069075:c647076-646810 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References