Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1433811..1434449 Replicon chromosome
Accession NZ_CP053601
Organism Escherichia coli BL21

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HO397_RS07050 Protein ID WP_000813794.1
Coordinates 1433811..1433987 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HO397_RS07055 Protein ID WP_001270286.1
Coordinates 1434033..1434449 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO397_RS07030 1429430..1430644 - 1215 WP_001326689.1 BenE family transporter YdcO -
HO397_RS07035 1430697..1431233 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HO397_RS07040 1431306..1433267 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HO397_RS07045 1433359..1433589 - 231 WP_000494244.1 YncJ family protein -
HO397_RS07050 1433811..1433987 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HO397_RS07055 1434033..1434449 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HO397_RS07060 1434528..1435934 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HO397_RS07065 1436179..1437324 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HO397_RS07070 1437342..1438355 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HO397_RS07075 1438356..1439297 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T157007 WP_000813794.1 NZ_CP053601:1433811-1433987 [Escherichia coli BL21]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T157007 NZ_CP069074:3208007-3208450 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT157007 WP_001270286.1 NZ_CP053601:1434033-1434449 [Escherichia coli BL21]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT157007 NZ_CP069074:3207763-3208020 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References