Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2873011..2873665 Replicon chromosome
Accession NZ_CP053600
Organism Escherichia coli strain Nico21(DE3)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HO398_RS13960 Protein ID WP_000244783.1
Coordinates 2873011..2873418 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HO398_RS13965 Protein ID WP_000354046.1
Coordinates 2873399..2873665 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO398_RS13940 2868968..2870701 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HO398_RS13945 2870707..2871417 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HO398_RS13950 2871442..2872338 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HO398_RS13955 2872450..2872971 + 522 WP_001055874.1 flavodoxin FldB -
HO398_RS13960 2873011..2873418 - 408 WP_000244783.1 protein YgfX Toxin
HO398_RS13965 2873399..2873665 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HO398_RS13970 2873908..2874888 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HO398_RS13975 2875084..2875743 - 660 WP_000250274.1 hemolysin III family protein -
HO398_RS13980 2875907..2876218 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HO398_RS13985 2876263..2877696 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
HO398_RS13990 2877753..2878496 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T156992 WP_000244783.1 NZ_CP053600:c2873418-2873011 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156992 NZ_CP069074:c2394175-2393741 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156992 WP_000354046.1 NZ_CP053600:c2873665-2873399 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156992 NZ_CP069074:c2394436-2394182 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References