Detailed information of TA system TA156970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4390595..4391409
• Replicon: chromosome
• Accession: NZ_CP053599
• Organism: Escherichia coli strain NEBExpress

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HO399_RS21135
• Protein ID: WP_001054376.1
• Coordinates: 4391152..4391409 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HO399_RS21130
• Protein ID: WP_001309181.1
• Coordinates: 4390595..4391140 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO399_RS21105	4386286..4387599	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HO399_RS21110	4387611..4387889	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HO399_RS21115	4387886..4389007	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HO399_RS21615	4389252..4389368	-	117	Protein_4110	VOC family protein	-
HO399_RS21120	4389406..4389624	-	219	Protein_4111	hypothetical protein	-
HO399_RS21125	4389793..4390539	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HO399_RS21130	4390595..4391140	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HO399_RS21135	4391152..4391409	-	258	WP_001054376.1	hypothetical protein	Toxin
HO399_RS21140	4391786..4392031	-	246	Protein_4115	GNAT family N-acetyltransferase	-
HO399_RS21145	4392401..4393387	+	987	Protein_4116	hypothetical protein	-
HO399_RS21150	4393970..4394950	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HO399_RS21155	4395015..4396121	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4382575..4403161	20586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T156970 WP_001054376.1 NZ_CP053599:c4391409-4391152 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T156970 NZ_CP069074:c760564-760127 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT156970 WP_001309181.1 NZ_CP053599:c4391140-4390595 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT156970 NZ_CP069074:c760815-760561 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8