Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3991008..3991845 Replicon chromosome
Accession NZ_CP053599
Organism Escherichia coli strain NEBExpress

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag HO399_RS19245 Protein ID WP_000227786.1
Coordinates 3991303..3991845 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HO399_RS19240 Protein ID WP_001297137.1
Coordinates 3991008..3991319 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO399_RS19215 3986028..3986975 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
HO399_RS19220 3986997..3988988 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HO399_RS19225 3988978..3989592 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HO399_RS19230 3989592..3989921 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HO399_RS19235 3989933..3990823 + 891 WP_000971336.1 heme o synthase -
HO399_RS19240 3991008..3991319 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HO399_RS19245 3991303..3991845 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
HO399_RS19250 3991901..3992836 - 936 WP_001297127.1 sel1 repeat family protein -
HO399_RS19255 3993253..3994617 + 1365 WP_001000978.1 MFS transporter -
HO399_RS19260 3994745..3995236 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HO399_RS19265 3995404..3996315 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T156967 WP_000227786.1 NZ_CP053599:3991303-3991845 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T156967 NZ_CP069074:c758159-757776 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATTAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTTGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT156967 WP_001297137.1 NZ_CP053599:3991008-3991319 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT156967 NZ_CP069074:c758409-758254 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References