Detailed information of TA system TA156965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2971359..2971997
• Replicon: chromosome
• Accession: NZ_CP053599
• Organism: Escherichia coli strain NEBExpress

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HO399_RS14145
• Protein ID: WP_000813794.1
• Coordinates: 2971821..2971997 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HO399_RS14140
• Protein ID: WP_001270286.1
• Coordinates: 2971359..2971775 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO399_RS14120	2966511..2967452	-	942	WP_001251313.1	ABC transporter permease	-
HO399_RS14125	2967453..2968466	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HO399_RS14130	2968484..2969629	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HO399_RS14135	2969874..2971280	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HO399_RS14140	2971359..2971775	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HO399_RS14145	2971821..2971997	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HO399_RS14150	2972219..2972449	+	231	WP_000494244.1	YncJ family protein	-
HO399_RS14155	2972541..2974502	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HO399_RS14160	2974575..2975111	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HO399_RS14165	2975164..2976378	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T156965 WP_000813794.1 NZ_CP053599:c2971997-2971821 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T156965 NZ_CP069074:720923-721318 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT156965 WP_001270286.1 NZ_CP053599:c2971775-2971359 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT156965 NZ_CP069074:720669-720923 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• PDB: 6HPB