Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2884807..2886395 Replicon chromosome
Accession NZ_CP053599
Organism Escherichia coli strain NEBExpress

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag HO399_RS13770 Protein ID WP_001125439.1
Coordinates 2885073..2886395 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag HO399_RS13765 Protein ID WP_001301023.1
Coordinates 2884807..2885073 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO399_RS13740 2879982..2880272 - 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -
HO399_RS13745 2880296..2881171 - 876 WP_000774188.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
HO399_RS13750 2881198..2882220 - 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
HO399_RS13755 2882232..2883008 - 777 Protein_2667 histidine kinase -
HO399_RS13760 2883004..2884584 + 1581 Protein_2668 autotransporter outer membrane beta-barrel domain-containing protein -
HO399_RS13765 2884807..2885073 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HO399_RS13770 2885073..2886395 + 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
HO399_RS13775 2886767..2887249 + 483 WP_000931227.1 fimbrial protein -
HO399_RS13780 2887610..2888320 + 711 Protein_2672 fimbria/pilus periplasmic chaperone -
HO399_RS13785 2888416..2888712 + 297 WP_174141747.1 hypothetical protein -
HO399_RS13790 2888667..2889800 + 1134 WP_001354080.1 fimbrial biogenesis outer membrane usher protein -
HO399_RS13795 2889864..2891012 + 1149 WP_000017447.1 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T156964 WP_001125439.1 NZ_CP053599:2885073-2886395 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T156964 NZ_CP069074:715265-715666 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT156964 WP_001301023.1 NZ_CP053599:2884807-2885073 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT156964 NZ_CP069074:715041-715268 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References