Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1528949..1529603 Replicon chromosome
Accession NZ_CP053599
Organism Escherichia coli strain NEBExpress

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HO399_RS07265 Protein ID WP_000244783.1
Coordinates 1528949..1529356 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HO399_RS07270 Protein ID WP_000354046.1
Coordinates 1529337..1529603 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO399_RS07245 1524906..1526639 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
HO399_RS07250 1526645..1527355 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HO399_RS07255 1527380..1528276 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HO399_RS07260 1528388..1528909 + 522 WP_001055874.1 flavodoxin FldB -
HO399_RS07265 1528949..1529356 - 408 WP_000244783.1 protein YgfX Toxin
HO399_RS07270 1529337..1529603 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HO399_RS07275 1529846..1530826 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HO399_RS07280 1531022..1531681 - 660 WP_000250274.1 hemolysin III family protein -
HO399_RS07285 1531845..1532156 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HO399_RS07290 1532201..1533634 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
HO399_RS07295 1533691..1534434 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T156954 WP_000244783.1 NZ_CP053599:c1529356-1528949 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156954 NZ_CP069074:71732-72133 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156954 WP_000354046.1 NZ_CP053599:c1529603-1529337 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156954 NZ_CP069074:71500-71739 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References