Detailed information of TA system TA156950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4393262..4394076
• Replicon: chromosome
• Accession: NZ_CP053597
• Organism: Escherichia coli strain T7Express_LysY

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HO400_RS21150
• Protein ID: WP_001054376.1
• Coordinates: 4393819..4394076 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HO400_RS21145
• Protein ID: WP_001309181.1
• Coordinates: 4393262..4393807 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO400_RS21120	4388953..4390266	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HO400_RS21125	4390278..4390556	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HO400_RS21130	4390553..4391674	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HO400_RS21135	4392073..4392291	-	219	Protein_4113	hypothetical protein	-
HO400_RS21140	4392460..4393206	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HO400_RS21145	4393262..4393807	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HO400_RS21150	4393819..4394076	-	258	WP_001054376.1	hypothetical protein	Toxin
HO400_RS21155	4394453..4394698	-	246	Protein_4117	GNAT family N-acetyltransferase	-
HO400_RS21160	4395068..4396054	+	987	Protein_4118	hypothetical protein	-
HO400_RS21165	4396637..4397617	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HO400_RS21170	4397682..4398788	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4393262..4403432	10170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T156950 WP_001054376.1 NZ_CP053597:c4394076-4393819 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T156950 NZ_CP069073:c3722145-3721717 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT156950 WP_001309181.1 NZ_CP053597:c4393807-4393262 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT156950 NZ_CP069073:c3722391-3722149 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8